Acuna is scheduled to hit leadoff and play right field against the Chicago Cubs on Thursday night.
The defending World Series champion Braves have struggled at the start of the season and are still looking for their first series win after splitting the first two games of their three-game series with the Cubs.
The Braves will be looking for Acuna to provide a boost to their lineup after he showed in his rehabilitation stint with Triple-A Gwinnett that he has recovered from the surgery that ended his 2021 season.
Acuna was 2-for-5 for Gwinnett on Wednesday night and hit .368 with three stolen bases in six games.
Despite the strong showing by Acuna in his rehabilitation assignment, the Braves had appeared firm in their plan to remain cautious ahead of his projected return on May 6.
There had been some signs that Acuna was influencing that plan, however. On Wednesday, manager Brian Snitker said the team would wait until after Acuna’s scheduled nine innings with Gwinnett on Thursday night before deciding on his next game.
The Braves’ decision to activate Acuna could have been motivated by losing Eddie Rosario for eight to 12 weeks. Rosario, the MVP of the NL Championship Series last season, had a laser procedure on Wednesday to correct blurred vision and swelling in his right eye.
The Braves designated outfielder Alex Dickerson for assignment to clear a spot for Acuna on the active roster.